Crystal structure of the amino-terminal domain of N-ethylmaleimide-sensitive fusion protein

Nat Cell Biol. 1999 Jul;1(3):175-82. doi: 10.1038/11097.


The cytosolic ATPase N-ethylmaleimide-sensitive fusion protein (NSF) disassembles complexes of membrane-bound proteins known as SNAREs, an activity essential for vesicular trafficking. The amino-terminal domain of NSF (NSF-N) is required for the interaction of NSF with the SNARE complex through the adaptor protein alpha-SNAP. The crystal structure of NSF-N reveals two subdomains linked by a single stretch of polypeptide. A polar interface between the two subdomains indicates that they can move with respect to one another during the catalytic cycle of NSF. Structure-based sequence alignments indicate that in addition to NSF orthologues, the p97 family of ATPases contain an amino-terminal domain of similar structure.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphatases / chemistry*
  • Adenosine Triphosphatases / drug effects
  • Amino Acid Sequence
  • Animals
  • Carrier Proteins / chemistry*
  • Carrier Proteins / drug effects
  • Carrier Proteins / metabolism*
  • Cloning, Molecular
  • Cricetinae
  • Cricetulus
  • Crystallography, X-Ray / methods
  • Ethylmaleimide / pharmacology*
  • Humans
  • Membrane Proteins / chemistry
  • Membrane Proteins / metabolism
  • Models, Molecular
  • Molecular Sequence Data
  • N-Ethylmaleimide-Sensitive Proteins
  • Peptide Fragments / chemistry
  • Protein Structure, Secondary
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / drug effects
  • Recombinant Proteins / metabolism
  • SNARE Proteins
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Vesicular Transport Proteins*


  • Carrier Proteins
  • Membrane Proteins
  • Peptide Fragments
  • Recombinant Proteins
  • SNARE Proteins
  • Vesicular Transport Proteins
  • Adenosine Triphosphatases
  • N-Ethylmaleimide-Sensitive Proteins
  • Ethylmaleimide