G1-phase and B-type cyclins exclude the DNA-replication factor Mcm4 from the nucleus

Nat Cell Biol. 1999 Nov;1(7):415-22. doi: 10.1038/15649.

Abstract

Cyclin-dependent kinases (CDKs) activate the firing of replication origins during the S phase of the cell cycle. They also block re-initiation of DNA replication within a single cell cycle, by preventing the assembly of prereplicative complexes at origins. We show here that, in budding yeast, CDKs exclude the essential prereplicative-complex component Mcm4 from the nucleus. Although origin firing can be triggered by the B-type cyclins only, both G1-phase and B-type cyclins cause exit of Mcm4 from the nucleus. These results suggest that G1 cyclins may diminish the cell's capacity to assemble prereplicative complexes before B-type cyclins trigger origin firing during S phase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CDC28 Protein Kinase, S cerevisiae / metabolism
  • Cell Cycle / physiology
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism*
  • Cell Nucleus / metabolism*
  • Chromatin / metabolism
  • Cyclin-Dependent Kinases / metabolism*
  • Cyclins / metabolism*
  • DNA Replication / genetics
  • DNA Replication / physiology
  • DNA-Binding Proteins*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • G1 Phase / physiology*
  • Genes, Reporter / genetics
  • Minichromosome Maintenance Complex Component 4
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • S Phase / physiology*
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / physiology
  • Saccharomyces cerevisiae Proteins*

Substances

  • CDC6 protein, S cerevisiae
  • Cell Cycle Proteins
  • Chromatin
  • Cyclins
  • DNA-Binding Proteins
  • Fungal Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • CDC28 Protein Kinase, S cerevisiae
  • Cyclin-Dependent Kinases
  • MCM4 protein, S cerevisiae
  • Minichromosome Maintenance Complex Component 4