Drosophila Lissencephaly-1 Functions With Bic-D and Dynein in Oocyte Determination and Nuclear Positioning

Nat Cell Biol. 1999 Nov;1(7):444-9. doi: 10.1038/15680.

Abstract

Here we show that the Drosophila homologue of Lissencephaly-1, DLis-1, acts together with Bicaudal-D (Bic-D), Egalitarian (Egl), dynein and microtubules to determine oocyte identity. DLis-1 is further required for nurse-cell-to-oocyte transport during oocyte growth, and for the positioning of the nucleus in the oocyte. Immunostaining of DLis-1 protein reveals a cortical localization that is independent of microtubules. DLis-1 may function in this position as a cortical anchor for the other nuclear-localization factors. DLis-1 and Bic-D are further required for nuclear localization in the developing nervous system, indicating that homologues of Bic-D, dynein and Egl-like proteins may also be involved in vertebrate neural migration and that their absence may cause a Miller-Dieker-like lissencephaly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Nucleus / metabolism*
  • Drosophila Proteins*
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / growth & development
  • Drosophila melanogaster / metabolism
  • Dyneins / metabolism*
  • Enhancer Elements, Genetic / genetics
  • Insect Proteins / metabolism*
  • Microscopy, Fluorescence
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism*
  • Microtubules / metabolism
  • Molecular Sequence Data
  • Neurons / physiology
  • Oocytes / physiology*
  • Oogenesis / genetics
  • Oogenesis / physiology
  • Photoreceptor Cells, Invertebrate / physiology
  • Sequence Alignment

Substances

  • BicD protein, Drosophila
  • Drosophila Proteins
  • Insect Proteins
  • Lis-1 protein, Drosophila
  • Microtubule-Associated Proteins
  • egl protein, Drosophila
  • Dyneins

Associated data

  • GENBANK/AF117606