Spinach hexokinase I is located in the outer envelope membrane of plastids

FEBS Lett. 1999 Nov 12;461(1-2):13-8. doi: 10.1016/s0014-5793(99)01417-9.


The subcellular localization of hexokinase activities in plant cells has been a matter of debate for a long time. We have isolated a hexokinase cDNA fragment from glucose-fed spinach leaves using a differential display reverse transcription-PCR approach. The corresponding cDNA was expressed in Escherichia coli and an antiserum, raised against the recombinant protein, was used in subcellular localization studies. The spinach hexokinase could be localized primarily to the outer envelope membrane of chloroplasts where it is inserted via its N-terminal membrane anchor. We suggest that the chloroplast envelope hexokinase is involved in the energization of glucose export from plastids rather than in the sugar-sensing pathway of the plant cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Western
  • Centrifugation, Density Gradient
  • Chloroplasts / enzymology
  • DNA, Complementary / analysis
  • Dose-Response Relationship, Drug
  • Escherichia coli / metabolism
  • Glucosamine / metabolism
  • Glucose / metabolism
  • Hexokinase / chemistry*
  • Hexokinase / genetics
  • Hexokinase / metabolism*
  • Molecular Sequence Data
  • Plant Proteins / metabolism
  • Plastids / enzymology*
  • Recombinant Proteins / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Spinacia oleracea / enzymology*
  • Sugar Phosphates / metabolism
  • Trehalose / analogs & derivatives
  • Trehalose / metabolism


  • DNA, Complementary
  • Plant Proteins
  • Recombinant Proteins
  • Sugar Phosphates
  • trehalose-6-phosphate
  • Trehalose
  • Hexokinase
  • Glucose
  • Glucosamine

Associated data

  • GENBANK/AF118132
  • GENBANK/AF118133