OTC and AUL1, two convergent and overlapping genes in the nuclear genome of Arabidopsis thaliana

FEBS Lett. 1999 Nov 12;461(1-2):101-6. doi: 10.1016/s0014-5793(99)01426-x.

Abstract

In contrast to bacterial, fungal and vertebrate ornithine transcarbamylases (OTCs; EC 2.1.3.3), very little is known about the enzyme in plants. We report here the isolation of a T-DNA-tagged mutant displaying sensitivity to ornithine, whose characterization has allowed for the identification of several complementary and genomic DNA clones encoding the OTC and auxilin-like 1 (AUL1) proteins of the crucifer Arabidopsis thaliana. Transcript mapping revealed that at least 22 bp within the OTC-AUL1 intercoding region are transcribed from both strands, which makes this one of the rarely described cases of convergent and overlapping transcription units in the nuclear genome of a multicellular eukaryote. Transcription of the OTC gene was shown to be ubiquitous in aerial organs of adult plants, whereas that of AUL1 was obscured by the existence of a putative second copy of the gene. The OTC-AUL1 locus maps at the bottom of chromosome 1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Vesicular Transport
  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis Proteins*
  • Base Sequence
  • Cell Nucleus / genetics*
  • DNA, Bacterial / genetics
  • DNA, Bacterial / metabolism
  • DNA, Complementary / metabolism
  • Genes, Overlapping
  • Genome, Plant
  • Models, Genetic
  • Molecular Sequence Data
  • Mutagenesis
  • Nerve Tissue Proteins / genetics*
  • Ornithine Carbamoyltransferase / genetics*
  • Phenotype
  • Phosphoproteins / genetics*
  • Phylogeny
  • Plant Proteins / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription, Genetic

Substances

  • Adaptor Proteins, Vesicular Transport
  • Arabidopsis Proteins
  • Aul1 protein, Arabidopsis
  • DNA, Bacterial
  • DNA, Complementary
  • Nerve Tissue Proteins
  • Phosphoproteins
  • Plant Proteins
  • T-DNA
  • Ornithine Carbamoyltransferase

Associated data

  • GENBANK/AJ000476
  • GENBANK/AJ002524
  • GENBANK/AJ007450