Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 34 (3), 607-19

A Response Regulator-Like Protein That Functions at an Intermediate Stage of Sporulation in Streptomyces Coelicolor A3(2)

Affiliations

A Response Regulator-Like Protein That Functions at an Intermediate Stage of Sporulation in Streptomyces Coelicolor A3(2)

J A Aínsa et al. Mol Microbiol.

Abstract

whiI is one of several loci originally described as essential for sporulation in Streptomyces coelicolor A3(2). We have characterized whiI at the molecular level. It encodes an atypical member of the response regulator family of proteins, lacking at least two of the residues strongly conserved in the conventional phosphorylation pocket. It is not adjacent to a potential sensor kinase gene. Fifteen mutant alleles of whiI were sequenced, revealing, among others, six mutations affecting conserved amino acids, several frameshift mutations and one mutation in the promoter. The whiI promoter is specifically transcribed by the sporulation-specific sigmaWhiG-containing form of RNA polymerase. Transcription of whiI is temporally controlled, reaching a maximum level coincident with the formation of spores. Further transcriptional studies suggested that WhiI is involved directly or indirectly in repressing its own expression and that of another sigmaWhiG-dependent sporulation-specific regulatory gene, whiH.

Similar articles

See all similar articles

Cited by 34 PubMed Central articles

See all "Cited by" articles

Publication types

MeSH terms

Substances

LinkOut - more resources

Feedback