Hydrogen peroxide-mediated Cu,Zn-superoxide dismutase fragmentation: protection by carnosine, homocarnosine and anserine

Biochim Biophys Acta. 1999 Nov 16;1472(3):651-7. doi: 10.1016/s0304-4165(99)00189-0.

Abstract

The fragmentation of human Cu,Zn-superoxide dismutase (SOD) was observed during incubation with H(2)O(2). Hydroxyl radical scavengers such as sodium azide, formate and mannitol protected the fragmentation of Cu,Zn-SOD. These results suggested that *OH was implicated in the hydrogen peroxide-mediated Cu,Zn-SOD fragmentation. Carnosine, homocarnosine and anserine have been proposed to act as anti-oxidants in vivo. We investigated whether three compounds could protect the fragmentation of Cu,Zn-SOD induced by H(2)O(2). The results showed that carnosine, homocarnosine and anserine significantly protected the fragmentation of Cu,Zn-SOD. All three compounds also protected the loss of enzyme activity induced by H(2)O(2). Carnosine, homocarnosine and anserine effectively inhibited the formation of *OH by the Cu,Zn-SOD/H(2)O(2) system. These results suggest that carnosine and related compounds can protect the hydrogen peroxide-mediated Cu,Zn-SOD fragmentation through the scavenging of *OH.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anserine / pharmacology
  • Carnosine / analogs & derivatives
  • Carnosine / pharmacology
  • Electrophoresis, Polyacrylamide Gel
  • Free Radical Scavengers / pharmacology*
  • Hydrogen Peroxide / chemistry*
  • Hydroxyl Radical
  • Pentetic Acid
  • Superoxide Dismutase / chemistry*

Substances

  • Free Radical Scavengers
  • Hydroxyl Radical
  • homocarnosine
  • Pentetic Acid
  • Carnosine
  • Hydrogen Peroxide
  • Superoxide Dismutase
  • Anserine