Increase in bone protein components with healing rat fractures: enhancement by zinc treatment

Int J Mol Med. 1999 Dec;4(6):615-20. doi: 10.3892/ijmm.4.6.615.


The alteration in bone components in the femoral-diaphyseal tissues with fracture healing was investigated. Rats were sacrificed 7 and 14 days after the femoral fracture. Protein content in the femoral-diaphyseal tissues was markedly elevated by fracture healing. Analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that many protein molecules were induced in the diaphyseal tissues with fracture healing. Moreover, when the femoral-diaphyseal tissues with fracture healing were cultured for 24 and 48 h in a serum-free medium, many proteins in the bone tissues were released into the medium. Also, the culture of the diaphyseal tissues with fracture healing caused a significant increase in bone alkaline phosphatase activity and deoxyribonucleic acid (DNA) content. Meanwhile, the presence of zinc acexamate (10-5 and 10-4 M), a stimulator of bone formation, in a culture medium induced a significant elevation of protein content and alkaline phosphatase activity in the diaphyseal tissues with fracture healing. Such an effect was completely abolished by the presence of cycloheximide (10-6 M), an inhibitor of protein synthesis. The present study suggests that fracture healing induces a newly synthesized bone protein component including stimulatory factor(s) for bone formation. Zinc supplementation may stimulate the healing of femoral fracture.

MeSH terms

  • Alkaline Phosphatase / analysis
  • Aminocaproates*
  • Aminocaproic Acid / pharmacology
  • Aminocaproic Acid / therapeutic use
  • Animals
  • Biomarkers
  • Cycloheximide / pharmacology
  • DNA / analysis
  • Femoral Fractures / drug therapy*
  • Femoral Fractures / metabolism
  • Fracture Healing / drug effects*
  • Male
  • Organ Culture Techniques
  • Osteoblasts / drug effects
  • Osteoporosis / prevention & control
  • Protein Biosynthesis
  • Protein Synthesis Inhibitors / pharmacology
  • Rats
  • Rats, Wistar


  • Aminocaproates
  • Biomarkers
  • Protein Synthesis Inhibitors
  • 6-acetylaminocaproic acid
  • DNA
  • Cycloheximide
  • Alkaline Phosphatase
  • Aminocaproic Acid