Cumulative prognostic value of p53 mutations and bcl-2 protein expression in head-and-neck cancer treated by radiotherapy

Int J Cancer. 1999 Dec 22;84(6):573-9. doi: 10.1002/(sici)1097-0215(19991222)84:6<573::aid-ijc6>;2-r.


We investigated the prognostic significance of p53-gene mutation (exon 5-9) and bcl-2-protein expression in primary squamous-cell carcinoma of the head and neck (HNSCC) treated by curative radiotherapy (RT). Primary squamous-cell carcinomas for analysis were obtained from 85 consecutive head-and-neck-cancer patients, with complete follow-up data. We detected bcl-2 protein in 24% (20/85) of HNSCC studied; 38 (45%) of the 85 tumours had cells bearing p53 mutations. A strong association was observed between tobacco exposure and bcl-2-protein expression (p = 0.003), an association also evident in those patients who had a p53-mutated carcinoma (p = 0.049). Moreover, we found that most of the bcl-2-positive cancers (70%) were also mutated in the p53 gene (p = 0.010). In univariate and in multivariate analyses, the simultaneous detection of bcl-2 expression and a p53-gene mutation in a tumour biopsy specimen was associated with greater risk of locoregional failure (p = 0.002 and 0.001 respectively) and worse survival (p = 0. 045 and 0.033) within 5 years in HNSCC patients treated by RT. The present study shows a cumulative prognostic value of simultaneous detection of bcl-2 over-expression and p53-gene aberration in some primary HNSCC treated with conventional RT, and provides further evidence for cross-talk between p53 and bcl-2, suggesting that these genes are important determinants of radiation-induced apoptosis, thereby modulating resistance to RT. Int. J. Cancer (Pred. Oncol.) 84:573-579, 1999.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Carcinoma, Squamous Cell / diagnosis*
  • Carcinoma, Squamous Cell / genetics*
  • Carcinoma, Squamous Cell / radiotherapy
  • Disease-Free Survival
  • Female
  • Genes, bcl-2 / genetics*
  • Genes, p53 / genetics*
  • Head and Neck Neoplasms / diagnosis*
  • Head and Neck Neoplasms / genetics*
  • Head and Neck Neoplasms / radiotherapy
  • Humans
  • Immunohistochemistry
  • Laryngeal Neoplasms / diagnosis
  • Laryngeal Neoplasms / genetics
  • Laryngeal Neoplasms / pathology
  • Laryngeal Neoplasms / radiotherapy
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Mutation
  • Prognosis
  • Proto-Oncogene Proteins c-bcl-2 / metabolism*
  • Survival Rate
  • Time Factors


  • Proto-Oncogene Proteins c-bcl-2