Mutation and transcription analyses of the p63 gene in cervical carcinoma

Int J Oncol. 1999 Dec;15(6):1149-53. doi: 10.3892/ijo.15.6.1149.


Genetic mutation of p53, which monitors DNA damage and operates cellular checkpoints, is a major factor in the development of human malignancies. A novel gene p63/p73L/p51, encoding a protein with significant homology to p53 and p73, was recently identified at 3q27-9. To investigate the penetration of p63 in cervical carcinogenesis, mutation and transcription analyses of p63 were performed in cervical carcinoma. A certain isotype of p63 called TAp63gamma encodes the acidic N-terminus and possesses a short C-terminus. Using reverse transcriptase-polymerase chain reaction-single strand conformation polymorphism (RT-PCR-SSCP) analysis for TAp63gamma, one mutation was found in the cervical carcinoma cell line SKG-I. However, no mutations causing amino acid substitutions or frameshifts were found in 54 cases examined for TAp63gamma, which is thought to be a tumor suppressor gene. While cervical carcinomas tended to yield a positive signal in the RT-PCR reaction designed to amplify transcripts encoding the acidic N-terminus, normal cervix and cervical intraepithelial neoplasia (CIN) did not express this transcript. These data suggest that the p63 gene does not play an essential role as a tumor suppressor gene, but expression of TAp63gamma may be speculatively associated with tumor growth in cervical carcinogenesis.

MeSH terms

  • Adenocarcinoma / genetics
  • Adenocarcinoma / pathology
  • Adult
  • Aged
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / pathology
  • DNA-Binding Proteins
  • Female
  • Gene Expression Regulation, Neoplastic
  • Genes, Tumor Suppressor / genetics*
  • HeLa Cells
  • Humans
  • Membrane Proteins*
  • Middle Aged
  • Mutation
  • Phosphoproteins / genetics*
  • Protein Isoforms / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Trans-Activators*
  • Transcription Factors
  • Transcription, Genetic
  • Tumor Cells, Cultured
  • Tumor Suppressor Proteins
  • Uterine Cervical Neoplasms / genetics*
  • Uterine Cervical Neoplasms / pathology


  • CKAP4 protein, human
  • DNA-Binding Proteins
  • Membrane Proteins
  • Phosphoproteins
  • Protein Isoforms
  • RNA, Messenger
  • TP63 protein, human
  • Trans-Activators
  • Transcription Factors
  • Tumor Suppressor Proteins