SCOP, a novel gene product expressed in a circadian manner in rat suprachiasmatic nucleus

FEBS Lett. 1999 Sep 24;458(3):363-9. doi: 10.1016/s0014-5793(99)01190-4.

Abstract

To elucidate the mechanism of the circadian rhythm, genes differentially expressed during subjective day and night in the rat suprachiasmatic nucleus (SCN), a circadian oscillator in mammals, were surveyed by a differential display method. We isolated a novel gene, scop (SCN circadian oscillatory protein), that was expressed in a circadian manner in the SCN. SCOP protein is predominantly expressed in the brain and has domains including a pleckstrin homology domain, leucine-rich repeats, a protein phosphatase 2C-like domain and a glutamine-rich region. The structural feature of SCOP protein suggests its role in the intracellular signaling in the SCN.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Circadian Rhythm
  • Cloning, Molecular
  • Gene Expression Regulation
  • Immunoblotting
  • Male
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / genetics*
  • Phosphoprotein Phosphatases
  • RNA, Messenger / genetics
  • Rats
  • Rats, Wistar
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Suprachiasmatic Nucleus / metabolism*

Substances

  • Nerve Tissue Proteins
  • Nuclear Proteins
  • RNA, Messenger
  • PHLPP1 protein, human
  • PHLPP1 protein, rat
  • Phosphoprotein Phosphatases

Associated data

  • GENBANK/AB023624