A novel calcitonin receptor gene in human osteoclasts from normal bone marrow

FEBS Lett. 1999 Sep 24;458(3):409-14. doi: 10.1016/s0014-5793(99)01176-x.


The calcitonin receptor (CTR) gene in human osteoclasts formed in a human bone marrow cell culture system was examined by reverse transcription-polymerase chain reaction (RT-PCR). The RT-PCR results indicated that the 5'-untranslated region (5'UTR) was different between CTR mRNAs in human osteoclasts and in a mammary tumor cell line, MCF-7 cells. We isolated the 5'UTR of the CTR gene from human osteoclasts, whose sequence had only 28.6% identity with that of other CTR genes reported until now. In a radioligand binding assay, COS-1 cells transfected with the osteoclast CTR gene bound to [125I]human CT (hCT). These results provided evidence that the CTR gene cloned from human osteoclasts was expressed functionally and its coding protein was identical to MCF-7 cell CTR.

MeSH terms

  • 5' Untranslated Regions / genetics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Bone Marrow / metabolism*
  • Breast Neoplasms
  • COS Cells
  • Cloning, Molecular
  • Humans
  • Molecular Sequence Data
  • Osteoclasts / metabolism*
  • Plasmids
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Calcitonin / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Transfection
  • Tumor Cells, Cultured


  • 5' Untranslated Regions
  • RNA, Messenger
  • Receptors, Calcitonin

Associated data

  • GENBANK/AB022177
  • GENBANK/AB022178