Human monocyte derived dendritic cells express functional P2X and P2Y receptors as well as ecto-nucleotidases

FEBS Lett. 1999 Sep 24;458(3):424-8. doi: 10.1016/s0014-5793(99)01197-7.


We investigated the expression and function of P2 receptors and ecto-nucleotidases on human monocyte derived dendritic cells (DC). In addition we analyzed the effect of extracellular ATP on the maturation of DC. By RT-PCR, DC were found to express mRNA for several P2X (P2X1, P2X4, P2X5, P2X7) and P2Y (P2Y1, P2Y2, P2Y4, P2Y5, P2Y6, P2Y10, P2Y11) receptors. As shown by FURA-2 measurement, triggering of P2 receptors resulted in an increase in free intracellular Ca2+. In combination with Tumor necrosis factor-alpha, ATP increased the expression of the DC surface markers CD80, CD83 and CD86 indicating a maturation promoting effect. DC expressed the ecto-apyrase CD39 and the ecto-5'-nucleotidase CD73 as demonstrated by RT-PCR. Extracellular ATP was rapidly hydrolyzed by these ecto-enzymes as shown by separation of 3H-labeled ATP metabolites using a thin layer technique. These data suggest that ATP acts as a costimulatory factor on DC maturation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5'-Nucleotidase / metabolism*
  • Adenosine Monophosphate / metabolism
  • Adenosine Triphosphate / metabolism
  • Adenosine Triphosphate / pharmacology
  • Antigens, CD / immunology
  • Antigens, Surface / immunology
  • Calcium / metabolism
  • Dendritic Cells / enzymology*
  • Fura-2
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
  • Humans
  • Interleukin-4 / pharmacology
  • Leukocytes, Mononuclear
  • RNA, Messenger / metabolism
  • Receptors, Purinergic P2 / genetics
  • Receptors, Purinergic P2 / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Necrosis Factor-alpha / pharmacology


  • Antigens, CD
  • Antigens, Surface
  • RNA, Messenger
  • Receptors, Purinergic P2
  • Tumor Necrosis Factor-alpha
  • Interleukin-4
  • Adenosine Monophosphate
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Adenosine Triphosphate
  • 5'-Nucleotidase
  • Calcium
  • Fura-2