Specific loss of chondromodulin-I gene expression in chondrosarcoma and the suppression of tumor angiogenesis and growth by its recombinant protein in vivo

FEBS Lett. 1999 Sep 24;458(3):436-40. doi: 10.1016/s0014-5793(99)01201-6.


Chondromodulin-I (ChM-I) was previously identified as an angiogenesis inhibitor in cartilage. Here, we demonstrated that the level of ChM-I transcripts was substantially reduced to 100 or even less in the lower-grade chondrosarcomas, in articular cartilage or other benign cartilage tumors. We implanted human chondrosarcoma OUMS-27 cells into nude mice that reproducibly produced tumors with cartilaginous matrix. Tumor-induced angiogenesis was evident when the tumors were excised 30 days after implantation. However, the local administration of recombinant human ChM-I almost completely blocked vascular invasion and tumor growth in vivo. Moreover, ChM-I also inhibited the growth of HT-29 colon adenocarcinoma in vivo, implying its therapeutic potential for solid tumors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / pharmacology
  • Bone Neoplasms / blood supply
  • Bone Neoplasms / genetics*
  • Bone Neoplasms / pathology
  • Cartilage / pathology
  • Chondrosarcoma / blood supply
  • Chondrosarcoma / genetics*
  • Chondrosarcoma / pathology
  • Gene Expression Regulation, Neoplastic
  • Growth Substances / analysis
  • Growth Substances / pharmacology*
  • HT29 Cells
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Membrane Proteins*
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Neovascularization, Pathologic / pathology*
  • RNA, Messenger / metabolism
  • Recombinant Proteins / pharmacology
  • Tumor Cells, Cultured


  • Antineoplastic Agents
  • Cnmd protein, mouse
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Membrane Proteins
  • RNA, Messenger
  • Recombinant Proteins
  • CNMD protein, human

Associated data

  • GENBANK/AB005999