BH-protocadherin-c, a member of the cadherin superfamily, interacts with protein phosphatase 1 alpha through its intracellular domain

FEBS Lett. 1999 Oct 22;460(1):93-8. doi: 10.1016/s0014-5793(99)01309-5.

Abstract

Using a yeast two-hybrid system, we isolated eight cDNA clones which interacted with BH-protocadherin-c (BH-Pcdh-c) from the human brain cDNA library. One clone encoded protein phosphatase type I isoform alpha (PP1alpha) and another two PP1alpha2. PP1alpha was co-immunoprecipitated from the extract of a gastric adenocarcinoma cell line MKN-28 with anti-BH-Pcdh-c antibody. PP1alpha activity towards glycogen phosphorylase was inhibited by the intracellular domain of BH-Pcdh-c. Inhibition of the phosphatase required more than the minimal domain of BH-Pcdh-c which could associate with PP1alpha. In situ hybridization revealed that BH-Pcdh-c mRNA was predominantly expressed in cerebral cortex neurons in the adult mouse brain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism
  • Cadherins / metabolism*
  • Cerebral Cortex / metabolism
  • Cloning, Molecular
  • Humans
  • In Situ Hybridization
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Oligonucleotides, Antisense
  • Phosphoprotein Phosphatases / antagonists & inhibitors
  • Phosphoprotein Phosphatases / metabolism*
  • Phosphorylases / metabolism
  • Precipitin Tests
  • Protein Binding
  • Protein Phosphatase 1
  • Protocadherins
  • RNA, Messenger / metabolism
  • Tumor Cells, Cultured
  • Yeasts

Substances

  • Cadherins
  • Oligonucleotides, Antisense
  • PCDH7 protein, human
  • Protocadherins
  • RNA, Messenger
  • Phosphorylases
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 1

Associated data

  • GENBANK/AB006757