Isolation and characterization of the E2F-like gene in plants

FEBS Lett. 1999 Oct 22;460(1):117-22. doi: 10.1016/s0014-5793(99)01296-x.

Abstract

The transcription factor E2F regulates the expression of genes involved in the progression of G1/S transition and DNA replication in mammalian cells. We cloned and characterized a cDNA (NtE2F) corresponding to a E2F homolog of tobacco (Nicotiana tabacum). The transcription of NtE2F was induced as cells progressed from G1 to the S phase and expressed much earlier than that of the proliferating cell nuclear antigen (PCNA) gene. We demonstrated that NtE2F can interact with the tobacco retinoblastoma (Rb)-related protein in a yeast two-hybrid assay. To further characterize NtE2F, the trans-activation activity of NtE2F was examined by using a transient assay in the tobacco Bright Yellow-2 (BY-2) cells with NtE2F fused to the DNA-binding domain of the veast transcriptional activator GAL4. NtE2F activated the transcription of the beta-glucuronidase (GUS) reporter gene driven by a cauliflower mosaic virus (CaMV) 35S core promoter containing the GAL4-binding sequence. This is the first report of the identification of a functionally equivalent E2F-like gene in plants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Carrier Proteins*
  • Cell Cycle Proteins / genetics
  • Cell Line
  • Cloning, Molecular
  • DNA-Binding Proteins*
  • E2F Transcription Factors
  • Gene Expression Regulation, Plant
  • Genes, Plant*
  • Genes, Reporter
  • Molecular Sequence Data
  • Plants, Toxic
  • Proliferating Cell Nuclear Antigen / genetics
  • RNA, Messenger / metabolism
  • Retinoblastoma Protein / genetics
  • Retinoblastoma-Binding Protein 1
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Tobacco
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*
  • Yeasts

Substances

  • Carrier Proteins
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • E2F Transcription Factors
  • Proliferating Cell Nuclear Antigen
  • RNA, Messenger
  • Retinoblastoma Protein
  • Retinoblastoma-Binding Protein 1
  • Transcription Factors

Associated data

  • GENBANK/AB025029
  • GENBANK/AB025347