Goalpha and diacylglycerol kinase negatively regulate the Gqalpha pathway in C. elegans

Neuron. 1999 Oct;24(2):323-33. doi: 10.1016/s0896-6273(00)80847-8.


We investigated the EGL-30 (Gqalpha) pathway in C. elegans by using genetic screens to identify genes that confer phenotypes similar to egl-30 mutants. One such gene, egl-8, encodes a phospholipase Cbeta that is present throughout the nervous system and near intestinal cell junctions. EGL-30 and EGL-8 appear to positively regulate synaptic transmission because reducing their function results in strong aldicarb resistance and slow locomotion rates. In contrast, GOA-1 (Goalpha) and DGK-1 (diacylglycerol kinase) appear to negatively regulate synaptic transmission, because reducing their function results in strong aldicarb hypersensitivity and hyperactive locomotion. A genetic analysis suggests that GOA-1 negatively regulates the EGL-30 pathway and that DGK-1 antagonizes the EGL-30 pathway.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / metabolism
  • Caenorhabditis elegans / physiology*
  • Diacylglycerol Kinase / physiology*
  • GTP-Binding Protein alpha Subunits, Gi-Go
  • Helminth Proteins / genetics
  • Helminth Proteins / physiology
  • Heterotrimeric GTP-Binding Proteins / metabolism
  • Heterotrimeric GTP-Binding Proteins / physiology*
  • Intestinal Mucosa / metabolism
  • Intestines / cytology
  • Isoenzymes / genetics
  • Molecular Sequence Data
  • Mutation / physiology
  • Nervous System / cytology
  • Nervous System / metabolism
  • Phenotype
  • Phospholipase C beta
  • Synaptic Transmission / physiology
  • Type C Phospholipases / genetics


  • Helminth Proteins
  • Isoenzymes
  • Diacylglycerol Kinase
  • Type C Phospholipases
  • Phospholipase C beta
  • GTP-Binding Protein alpha Subunits, Gi-Go
  • Heterotrimeric GTP-Binding Proteins

Associated data

  • GENBANK/AF179426