Abstract
Many respiratory isolates of Pseudomonas aeruginosa from cystic fibrosis patients are mucoid (alginate producing) yet lack flagella. It was hypothesized that an alginate regulator inhibits flagellar gene expression. Mutations in algB, algR, and algT resulted in nonmucoid derivatives, yet algT mutants expressed flagella. AlgT-dependent control of flagellum synthesis occurred through inhibition of fliC but not rpoN transcription.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alginates / metabolism
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Bacterial Proteins / genetics
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Bacterial Proteins / physiology*
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Carbohydrate Dehydrogenases / genetics
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Catechol 2,3-Dioxygenase
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DNA-Binding Proteins*
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Dioxygenases*
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Flagella / genetics
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Flagella / metabolism*
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Flagellin / genetics
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Gene Expression Regulation, Bacterial
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Microscopy, Electron
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Movement
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Mutation
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Oxygenases / genetics
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Phenotype
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Pseudomonas aeruginosa / genetics
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Pseudomonas aeruginosa / metabolism*
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Pseudomonas aeruginosa / physiology
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Sigma Factor / metabolism*
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Trans-Activators*
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Transcription Factors / genetics
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Transcription, Genetic
Substances
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AlgR protein, Pseudomonas aeruginosa
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AlgU protein, Pseudomonas aeruginosa
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Alginates
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Bacterial Proteins
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DNA-Binding Proteins
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Sigma Factor
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Trans-Activators
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Transcription Factors
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Flagellin
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AlgB protein, Pseudomonas
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Carbohydrate Dehydrogenases
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GDPmannose dehydrogenase
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Oxygenases
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Dioxygenases
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Catechol 2,3-Dioxygenase