Synergistic effect of angiotensin-(1-7) on bradykinin arteriolar dilation in vivo

Peptides. 1999;20(10):1195-201. doi: 10.1016/s0196-9781(99)00123-0.

Abstract

The interaction between angiotensin [Ang-(1-7)] and bradykinin (BK) was determined in the mesentery of anesthetized Wistar rats using intravital microscopy. Topical application of BK and Ang-(1-7) induced vasodilation that was abolished by the BK B2 receptor antagonist HOE-140 and the Ang-(1-7) antagonist A-779, respectively. BK (1 pmol)-induced vasodilation, but not SNP and ACh responses, was potentiated by Ang-(1-7) 10 pmol and 100 pmols. The effect of 100 pmol of Ang-(1-7) on BK-induced vasodilation was abolished by A-779, indomethacin, and L-nitroarginine methyl esther, whereas losartan was without effect. Enalaprilat treatment enhanced the BK- and Ang-(1-7)-induced vasodilation and the potentiating effect of Ang-(1-7) on BK vasodilation. The potentiation of BK-induced vasodilation by Ang-(1-7) is a receptor-mediated phenomenon dependent on cyclooxygenase-related products and NO release.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin I
  • Angiotensin II / analogs & derivatives
  • Angiotensin II / metabolism
  • Angiotensin II / pharmacology*
  • Angiotensin-Converting Enzyme Inhibitors / pharmacology
  • Animals
  • Arterioles / drug effects
  • Arterioles / physiology
  • Bradykinin / analogs & derivatives
  • Bradykinin / metabolism
  • Bradykinin / pharmacology*
  • Bradykinin Receptor Antagonists
  • Drug Synergism
  • Enalaprilat / pharmacology
  • Mesenteric Arteries / drug effects
  • Nitric Oxide / metabolism
  • Peptide Fragments / metabolism
  • Peptide Fragments / pharmacology*
  • Prostaglandins / metabolism
  • Rats
  • Rats, Wistar
  • Receptor, Bradykinin B2
  • Vasodilation / drug effects
  • Vasodilator Agents / metabolism
  • Vasodilator Agents / pharmacology*

Substances

  • 7-Ala-angiotensin (1-7)
  • Angiotensin-Converting Enzyme Inhibitors
  • Bradykinin Receptor Antagonists
  • Peptide Fragments
  • Prostaglandins
  • Receptor, Bradykinin B2
  • Vasodilator Agents
  • Angiotensin II
  • Nitric Oxide
  • icatibant
  • Angiotensin I
  • Enalaprilat
  • angiotensin I (1-7)
  • Bradykinin