Targeted integration of a dominant neo(R) marker into a 2- to 3-Mb human minichromosome and transfer between cells

Cytogenet Cell Genet. 1999;86(3-4):194-203. doi: 10.1159/000015338.

Abstract

The physical and genetic characterization of a stable human minichromosome in a Chinese hamster hybrid cell is described. The minichromosome is 2-3 Mb in size, is linear, and contains a complementing SDHC gene. It is derived from a human chromosome 1, including the centromere, some pericentric heterochromatin from 1q12, and 1-2 Mb of 1q21. Genomic DNA surrounding the SDHC gene was used to construct a targeting vector with a selectable drug resistance marker (neo(R)); the marker was then successfully integrated into the minichromosome. With the new selectable marker, the 8.2.3 minichromosome could be transferred into mouse LMTK(-) and 3T3 TK(-) cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cardiopulmonary Resuscitation
  • Cell Line
  • Chromosome Mapping
  • Chromosomes, Human*
  • Chromosomes, Human, Pair 1*
  • Cricetinae
  • Cricetulus
  • Electrophoresis, Gel, Pulsed-Field
  • Gene Transfer Techniques
  • Humans
  • Hybrid Cells
  • In Situ Hybridization, Fluorescence
  • Karyotyping
  • L Cells
  • Mice
  • Succinate Dehydrogenase / genetics*
  • Thymidine Kinase / deficiency
  • Thymidine Kinase / genetics

Substances

  • Succinate Dehydrogenase
  • Thymidine Kinase