In vitro inducible nitric oxide synthesis inhibitory active constituents from Fraxinus rhynchophylla

Planta Med. 1999 Oct;65(7):656-8. doi: 10.1055/s-2006-960840.

Abstract

Bioassay-guided fractionation of an H2O extract of the barks of Fraxinus rhynchophylla has furnished two inducible nitric oxide synthase (iNOS) inhibitory compounds, ferulaldehyde (1) and scopoletin (3) together with a coumarin, fraxidin (2). Compounds 1 and 3 showed inhibition of nitric oxide (NO) synthesis in a dose-dependent manner by murine macrophage-like RAW 264.7 cells stimulated with interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS). The inhibition of NO synthesis of 1 was reflected in the decreased amount of iNOS protein, as determined by Western blotting.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrolein / analogs & derivatives*
  • Acrolein / isolation & purification
  • Acrolein / pharmacology
  • Animals
  • Cell Line
  • Coumarins / isolation & purification
  • Coumarins / pharmacology*
  • Enzyme Inhibitors / isolation & purification
  • Enzyme Inhibitors / pharmacology*
  • Nitric Oxide / antagonists & inhibitors*
  • Nitric Oxide / biosynthesis
  • Nitric Oxide Synthase / antagonists & inhibitors*
  • Nitric Oxide Synthase Type II
  • Plants, Medicinal / chemistry*
  • Scopoletin / isolation & purification
  • Scopoletin / pharmacology*

Substances

  • Coumarins
  • Enzyme Inhibitors
  • fraxiden
  • coniferaldehyde
  • Nitric Oxide
  • Acrolein
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type II
  • Scopoletin