Abstract
Bioassay-guided fractionation of an H2O extract of the barks of Fraxinus rhynchophylla has furnished two inducible nitric oxide synthase (iNOS) inhibitory compounds, ferulaldehyde (1) and scopoletin (3) together with a coumarin, fraxidin (2). Compounds 1 and 3 showed inhibition of nitric oxide (NO) synthesis in a dose-dependent manner by murine macrophage-like RAW 264.7 cells stimulated with interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS). The inhibition of NO synthesis of 1 was reflected in the decreased amount of iNOS protein, as determined by Western blotting.
Publication types
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Letter
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Research Support, Non-U.S. Gov't
MeSH terms
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Acrolein / analogs & derivatives*
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Acrolein / isolation & purification
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Acrolein / pharmacology
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Animals
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Cell Line
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Coumarins / isolation & purification
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Coumarins / pharmacology*
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Enzyme Inhibitors / isolation & purification
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Enzyme Inhibitors / pharmacology*
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Nitric Oxide / antagonists & inhibitors*
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Nitric Oxide / biosynthesis
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Nitric Oxide Synthase / antagonists & inhibitors*
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Nitric Oxide Synthase Type II
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Plants, Medicinal / chemistry*
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Scopoletin / isolation & purification
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Scopoletin / pharmacology*
Substances
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Coumarins
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Enzyme Inhibitors
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fraxiden
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coniferaldehyde
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Nitric Oxide
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Acrolein
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Nitric Oxide Synthase
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Nitric Oxide Synthase Type II
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Scopoletin