Regulation of developmental senescence is conserved between Arabidopsis and Brassica napus

Plant Mol Biol. 1999 Sep;41(2):195-206. doi: 10.1023/a:1006389803990.


SAG12 is a developmentally controlled, senescence-specific gene from Arabidopsis which encodes a cysteine protease. Using SAG12 as a probe, we isolated two SAG12 homologues (BnSAG12-1 and BnSAG12-2) from Brassica napus. Structural comparisons and expression studies indicate that these two genes are orthologues of SAG12. The expression patterns of BnSAG12-1 and BnSAG12-2 in Arabidopsis demonstrate that the senescence-specific regulation of this class of cysteine proteases is conserved across these species. Gel-shift assays using the essential promoter regions of SAG12, BnSAG12-1, and BnSAG12-2 show that the extent of binding of a senescence-specific, DNA-binding protein from Arabidopsis is proportional to the expression levels of these genes in Arabidopsis. Therefore, the expression levels of these genes may reflect the affinities of the senescence-specific DNA-binding protein for the promoter element.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / enzymology
  • Arabidopsis / genetics*
  • Arabidopsis Proteins*
  • Base Sequence
  • Brassica / enzymology
  • Brassica / genetics*
  • Cysteine Endopeptidases / genetics*
  • DNA, Plant
  • DNA-Binding Proteins / metabolism
  • Evolution, Molecular*
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Plant
  • Genome, Plant
  • Genomic Library
  • Molecular Sequence Data
  • Plant Proteins*
  • Sequence Homology, Amino Acid


  • Arabidopsis Proteins
  • DNA, Plant
  • DNA-Binding Proteins
  • Plant Proteins
  • SAG12 protein, Arabidopsis
  • Cysteine Endopeptidases

Associated data

  • GENBANK/AF089848
  • GENBANK/AF089849