Free thiol groups are essential for infectivity of human cytomegalovirus

J Gen Virol. 1999 Nov;80 ( Pt 11):2861-2865. doi: 10.1099/0022-1317-80-11-2861.

Abstract

The membrane-impermeable thiol blocker 5'5-dithiobis 2- nitrobenzoic acid (DTNB) blocked infectivity of human cytomegalovirus (CMV) although the virus still bound to cells. DTNB-treated CMV regained 65% of its infectivity after incubation with the disulfide bond-reducing agent dithiothreitol. These observations suggest that free thiol groups on CMV are required for infectivity and may participate in disulfide bond formation during virus entry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cytomegalovirus / drug effects
  • Cytomegalovirus / pathogenicity*
  • Dithionitrobenzoic Acid / pharmacology
  • Dithiothreitol / pharmacology
  • Humans
  • Sulfhydryl Compounds / physiology*
  • Viral Envelope Proteins / physiology

Substances

  • Sulfhydryl Compounds
  • Viral Envelope Proteins
  • glycoprotein B, Simplexvirus
  • Dithionitrobenzoic Acid
  • Dithiothreitol