Pseudotyping human immunodeficiency virus type 1 by vesicular stomatitis virus G protein does not reduce the cell-dependent requirement of vif for optimal infectivity: functional difference between Vif and Nef

J Gen Virol. 1999 Nov;80 ( Pt 11):2945-2949. doi: 10.1099/0022-1317-80-11-2945.

Abstract

The functions of Vif and Nef in human immunodeficiency virus type 1 (HIV-1) infection have some similarities: Vif- and Nef-dependent enhancement of HIV-1 replication is cell type-specific, and defective mutations in these genes result in restricted proviral DNA synthesis in infected cells. It has recently been shown that pseudotyping HIV-1 by the envelope glycoprotein of vesicular stomatitis virus (VSV-G) targets HIV-1 entry to an endocytic pathway and suppresses the requirement of Nef for virus infectivity. In this study, we examined whether VSV-G pseudotyping suppresses the requirement of Vif for HIV-1 infectivity. It was found that pseudotyping HIV-1 by VSV-G did not compensate for the Vif function. Together with the findings that Vif does not influence virus binding/entry and virion incorporation of Env, it is concluded that Vif enhances HIV-1 infectivity at the post-entry step(s) independently of the Env function by a different mechanism to that of Nef.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Products, nef / physiology*
  • Gene Products, vif / physiology*
  • HIV-1 / physiology*
  • Humans
  • Membrane Glycoproteins*
  • Viral Envelope Proteins / physiology*
  • Virion / physiology
  • nef Gene Products, Human Immunodeficiency Virus
  • vif Gene Products, Human Immunodeficiency Virus

Substances

  • G protein, vesicular stomatitis virus
  • Gene Products, nef
  • Gene Products, vif
  • Membrane Glycoproteins
  • Viral Envelope Proteins
  • nef Gene Products, Human Immunodeficiency Virus
  • vif Gene Products, Human Immunodeficiency Virus