B7-H1, a Third Member of the B7 Family, Co-Stimulates T-cell Proliferation and interleukin-10 Secretion

Nat Med. 1999 Dec;5(12):1365-9. doi: 10.1038/70932.

Abstract

The B7 family members B7-1 and B7-2 interact with CD28 and constitute an essential T-cell co-stimulatory pathway in the initiation of antigen-specific humoral and cell-mediated immune response. Here, we describe a third member of the B7 family, called B7-H1 that does not bind CD28, cytotoxic T-lymphocyte A4 or ICOS (inducible co-stimulator). Ligation of B7-H1 co-stimulated T-cell responses to polyclonal stimuli and allogeneic antigens, and preferentially stimulated the production of interleukin-10. Interleukin-2, although produced in small amounts, was required for the effect of B7-H1 co-stimulation. Our studies thus define a previously unknown co-stimulatory molecule that may be involved in the negative regulation of cell-mediated immune responses.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • B7-1 Antigen / genetics
  • B7-1 Antigen / immunology*
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Gene Expression
  • Humans
  • Immunity, Cellular
  • In Vitro Techniques
  • Interleukin-10 / biosynthesis
  • Interleukin-10 / metabolism*
  • Interleukin-2 / biosynthesis
  • Lymphocyte Activation
  • Molecular Sequence Data
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Sequence Homology, Amino Acid
  • T-Lymphocytes / immunology*
  • Transfection

Substances

  • B7-1 Antigen
  • DNA, Complementary
  • Interleukin-2
  • Recombinant Proteins
  • Interleukin-10

Associated data

  • GENBANK/AF177937