Striatum- and testis-specific phosphodiesterase PDE10A isolation and characterization of a rat PDE10A

Eur J Biochem. 1999 Dec;266(3):1118-27. doi: 10.1046/j.1432-1327.1999.00963.x.


PDE10A, a phosphodiesterase (PDE) exhibiting properties of a cAMP PDE and a cAMP-inhibited cGMP PDE, was cloned and investigated in detail in rats. PDE10A transcripts were abundant in the brain and testis. In situ hybridization analysis using a PDE10A riboprobe demonstrated the presence of PDE10A transcripts in the neurons of the striatum and the olfactory tubercle regions of the brain. Rat PDE10A cDNAs were isolated from a brain cDNA library and nucleotide sequence analysis revealed several N-terminal variants. The deduced amino-acid sequence of one of the major variant forms contained 794 amino acids, and it was 96% identical to that of the human PDE10A2. The other major form has a distinct N-terminal sequence that is not found in humans. PDE10A was partially purified from rat striatum and testis, and characterized with respect to Km, inhibitor sensitivity and immunoreactivity to an anti-PDE10A serum. These findings indicate that PDE10A functions in these tissues.

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • COS Cells
  • Chromatography, Ion Exchange
  • Cloning, Molecular
  • Corpus Striatum / enzymology*
  • DNA Primers / genetics
  • DNA, Complementary / genetics
  • Genetic Variation
  • Humans
  • In Situ Hybridization
  • Kinetics
  • Male
  • Molecular Sequence Data
  • Phosphoric Diester Hydrolases / genetics*
  • Phosphoric Diester Hydrolases / isolation & purification*
  • Phosphoric Diester Hydrolases / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Sequence Homology, Amino Acid
  • Testis / enzymology*
  • Tissue Distribution
  • Transfection


  • DNA Primers
  • DNA, Complementary
  • RNA, Messenger
  • Recombinant Proteins
  • PDE10A protein, human
  • Phosphoric Diester Hydrolases

Associated data

  • GENBANK/AB027155
  • GENBANK/AB027156