Enzymatic function of the nor-1 protein in aflatoxin biosynthesis in Aspergillus parasiticus

Appl Environ Microbiol. 1999 Dec;65(12):5639-41. doi: 10.1128/AEM.65.12.5639-5641.1999.

Abstract

The nor-1 gene is involved in aflatoxin biosynthesis in Aspergillus parasiticus and was predicted to encode a norsolorinic acid ketoreductase. Recombinant Nor-1 expressed in Escherichia coli converted the 1' keto group of norsolorinic acid to the 1' hydroxyl group of averantin in crude E. coli cell extracts in the presence of NADPH. The results confirm that Nor-1 functions as a ketoreductase in vitro.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aflatoxins / biosynthesis*
  • Alcohol Dehydrogenase / isolation & purification
  • Alcohol Dehydrogenase / metabolism*
  • Alcohol Oxidoreductases / isolation & purification
  • Alcohol Oxidoreductases / metabolism*
  • Aspergillus / enzymology*
  • Cloning, Molecular
  • Escherichia coli
  • Fungal Proteins*
  • NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism

Substances

  • Aflatoxins
  • Fungal Proteins
  • Recombinant Proteins
  • Alcohol Oxidoreductases
  • NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases
  • Alcohol Dehydrogenase
  • norsolorinic acid ketoreductase