Sequence analysis and gene identification in a set of mapped RFLP markers in barley (Hordeum vulgare)

Genome. 1999 Oct;42(5):849-53. doi: 10.1139/g99-036.


The "Igri/Franka" (I/F) map ranks among the most comprehensive genetic linkage maps of barley (Hordeum vulgare), containing a large number of markers derived from cDNA and genomic PstI clones. Fourty-three cDNA clones and 259 genomic clones were at least partially sequenced and compared with the major data bases of protein and nucleic acid sequences. Of the cDNA clones, 53% show significant similarity to known sequences in protein data bases. A comparison of sequences from genomic clones to nucleic acid sequence data bases revealed similarities for 9% of the clones. For cDNA sequences analyzed the same way, significant similarities were observed for 35% of the clones. These results show that genomic PstI clones, although containing genes at a significant frequency, represent an inappropriate source for an efficient, systematic gene identification in barley. Sequence information obtained in the context of the present study provides a resource for the conversion of these markers into sequence-tagged site (STS) markers and their use in PCR assays.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Complementary / analysis
  • DNA, Plant / analysis
  • Genes, Plant*
  • Genetic Linkage
  • Genome, Plant
  • Hordeum / genetics*
  • Polymorphism, Restriction Fragment Length
  • Sequence Alignment
  • Sequence Analysis, DNA


  • DNA, Complementary
  • DNA, Plant

Associated data

  • GENBANK/AJ234400
  • GENBANK/AJ234401
  • GENBANK/AJ234402
  • GENBANK/AJ234403
  • GENBANK/AJ234404
  • GENBANK/AJ234405
  • GENBANK/AJ234406
  • GENBANK/AJ234407
  • GENBANK/AJ234408
  • GENBANK/AJ234409
  • GENBANK/AJ234410
  • GENBANK/AJ234411
  • GENBANK/AJ234412
  • GENBANK/AJ234413
  • GENBANK/AJ234414
  • GENBANK/AJ234415
  • GENBANK/AJ234416
  • GENBANK/AJ234417
  • GENBANK/AJ234418
  • GENBANK/AJ234419
  • GENBANK/AJ234420
  • GENBANK/AJ234421
  • GENBANK/AJ234422
  • GENBANK/AJ234423
  • GENBANK/AJ234424
  • GENBANK/AJ234425
  • GENBANK/AJ234426
  • GENBANK/AJ234427
  • GENBANK/AJ234428
  • GENBANK/AJ234429