Semiquantitative polymerase chain reaction enzyme immunoassay for the diagnosis of pertussis

Eur J Clin Microbiol Infect Dis. 1999 Oct;18(10):748-50. doi: 10.1007/s100960050392.

Abstract

The two most commonly used targets for diagnosis of pertussis by the polymerase chain reaction have been the pertussis toxin promoter and the repeated insertion sequence IS481. A comparative assessment of these primers was performed on routinely collected nasopharyngeal swabs, stored at -20 C, using novel semiquantitative enzyme immunoassays. Both sets of primers behaved similarly with bacterial suspensions, and the 17 culture-positive nasopharyngeal swabs were also positive with the pertussis toxin promoter primers, with one exception, which had been subject to prolonged storage. Significantly more of the 69 culture-negative swabs were positive with the pertussis toxin promoter primers (n = 36) than with the IS481 primers (n = 18). To determine the effect of inhibitors, a comparative assessment of three primer pairs against human DNA (beta-globin and glyceraldehyde-3-phosphate dehydrogenase) was also performed.

MeSH terms

  • Child
  • DNA / analysis
  • Humans
  • Immunoenzyme Techniques
  • Nasopharynx / microbiology*
  • Polymerase Chain Reaction / methods*
  • Whooping Cough / diagnosis*

Substances

  • DNA