DFak56 is a novel Drosophila melanogaster focal adhesion kinase

J Biol Chem. 1999 Dec 10;274(50):35621-9. doi: 10.1074/jbc.274.50.35621.


The mammalian focal adhesion kinase (FAK) family of nonreceptor protein-tyrosine kinases have been implicated in controlling a multitude of cellular responses to the engagement of cell surface integrins and G protein-coupled receptors. We describe here a Drosophila melanogaster FAK homologue, DFak56, which maps to band 56D on the right arm of the second chromosome. Full-length DFak56 cDNA encodes a phosphoprotein of 140 kDa, which shares strong sequence similarity not only with mammalian p125(FAK) but also with the more recently described mammalian Pyk2 (also known as CAKbeta, RAFTK, FAK2, and CADTK) FAK family member. DFak56 has intrinsic tyrosine kinase activity and is phosphorylated on tyrosine in vivo. As is the case for FAK, tyrosine phosphorylation of DFak56 is increased upon plating Drosophila embryo cells on extracellular matrix proteins. In situ hybridization and immunofluorescence staining analysis showed that DFak56 is ubiquitously expressed with particularly high levels within the developing central nervous system. We utilized the UAS-GAL4 expression system to express DFak56 and analyze its function in vivo. Overexpression of DFak56 in the wing imaginal disc results in wing blistering in adults, a phenotype also observed with both position-specific integrin loss of function and position-specific integrin overexpression. Our results imply a role for DFak56 in adhesion-dependent signaling pathways in vivo during D. melanogaster development.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Adhesion Molecules / chemistry
  • Cell Adhesion Molecules / genetics*
  • Cell Adhesion Molecules / metabolism*
  • Chromosome Mapping*
  • DNA, Complementary
  • Drosophila Proteins
  • Drosophila melanogaster / enzymology*
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / growth & development
  • Embryo, Nonmammalian
  • Evolution, Molecular
  • Focal Adhesion Kinase 1
  • Focal Adhesion Protein-Tyrosine Kinases
  • Gene Expression Regulation, Developmental*
  • Gene Expression Regulation, Enzymologic
  • Larva
  • Mammals
  • Molecular Sequence Data
  • Molecular Weight
  • Phylogeny
  • Protein-Tyrosine Kinases / chemistry
  • Protein-Tyrosine Kinases / genetics*
  • Protein-Tyrosine Kinases / metabolism*
  • Pupa
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • src Homology Domains


  • Cell Adhesion Molecules
  • DNA, Complementary
  • Drosophila Proteins
  • Recombinant Proteins
  • Protein-Tyrosine Kinases
  • Fak protein, Drosophila
  • Focal Adhesion Kinase 1
  • Focal Adhesion Protein-Tyrosine Kinases

Associated data

  • GENBANK/AF112116