Reevaluation of the promoter structure of the class 3 flagellar operons of Escherichia coli and Salmonella

Genes Genet Syst. 1999 Jun;74(3):113-6. doi: 10.1266/ggs.74.113.

Abstract

Flagellar class 3 operons of Escherichia coli and Salmonella are transcribed by RNA polymerase containing sigma 28. The consensus sequence of the sigma 28-dependent promoters was believed to be TAAA N15 GCCGATAA. In this study, we found that the E. coli genome contains a large number of sequences homologous to this consensus. However, we showed that they do not always exert a sigma 28-dependent promoter activity. We compare more carefully the sequences of the class 3 flagellar promoters and propose a revised structure of the sigma 28-dependent promoters as TAAAGTTT N11 GCCGATAA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA-Directed RNA Polymerases / genetics*
  • Databases, Factual
  • Escherichia coli / genetics*
  • Molecular Sequence Data
  • Operon / genetics*
  • Promoter Regions, Genetic*
  • Salmonella / genetics*
  • Sequence Homology, Nucleic Acid

Substances

  • RNA polymerase sigma(28)
  • DNA-Directed RNA Polymerases