Large-scale analysis of the yeast genome by transposon tagging and gene disruption

Nature. 1999 Nov 25;402(6760):413-8. doi: 10.1038/46558.


Economical methods by which gene function may be analysed on a genomic scale are relatively scarce. To fill this need, we have developed a transposon-tagging strategy for the genome-wide analysis of disruption phenotypes, gene expression and protein localization, and have applied this method to the large-scale analysis of gene function in the budding yeast Saccharomyces cerevisiae. Here we present the largest collection of defined yeast mutants ever generated within a single genetic background--a collection of over 11,000 strains, each carrying a transposon inserted within a region of the genome expressed during vegetative growth and/or sporulation. These insertions affect nearly 2,000 annotated genes, representing about one-third of the 6,200 predicted genes in the yeast genome. We have used this collection to determine disruption phenotypes for nearly 8,000 strains using 20 different growth conditions; the resulting data sets were clustered to identify groups of functionally related genes. We have also identified over 300 previously non-annotated open reading frames and analysed by indirect immunofluorescence over 1,300 transposon-tagged proteins. In total, our study encompasses over 260,000 data points, constituting the largest functional analysis of the yeast genome ever undertaken.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Algorithms
  • DNA Transposable Elements*
  • Escherichia coli / genetics
  • Fungal Proteins / genetics
  • Fungal Proteins / physiology
  • Gene Expression Profiling
  • Genetic Techniques*
  • Genome, Fungal*
  • Molecular Sequence Data
  • Mutagenesis
  • Oligonucleotide Array Sequence Analysis
  • Open Reading Frames
  • Phenotype
  • Polymerase Chain Reaction
  • Saccharomyces cerevisiae / genetics*
  • Transformation, Genetic


  • DNA Transposable Elements
  • Fungal Proteins

Associated data

  • GENBANK/U54828