The effect of corticosteroids on amyloid beta precursor protein/amyloid precursor-like protein expression and processing in vivo

Neurosci Lett. 1999 Nov 26;276(1):61-4. doi: 10.1016/s0304-3940(99)00790-9.

Abstract

In this study, we have investigated the effect of altered corticosteroid levels on the expression and processing of the amyloid beta precursor protein (A betaPP) and its amyloid precursor-like protein (APLP) homologue in rat brain. Four groups of animals were used in the study: sham operated, adrenalectomised, and adrenalectomised treated with either dexamethasone or aldosterone, with the A betaPP/APLP expression being determined by western blot analysis. While there were no changes in the levels of A betaPP/APLP following adrenalectomy, treatment with dexamethasone, but not aldosterone, resulted in a marked increase in protein expression levels with the level of increase varying between the brain regions examined. Corticosteroids had a more marked effect on the particulate rather than the soluble form of the protein, thus suggesting that elevated glucocorticoids may also be adversely influencing A betaPP/APLP processing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Cortex Hormones / pharmacology
  • Adrenal Cortex Hormones / physiology*
  • Adrenalectomy
  • Aldosterone / pharmacology
  • Amyloid beta-Protein Precursor / analogs & derivatives*
  • Amyloid beta-Protein Precursor / metabolism*
  • Animals
  • Blotting, Western
  • Brain / drug effects
  • Brain / metabolism*
  • Dexamethasone / pharmacology
  • Glucocorticoids / pharmacology
  • Rats
  • Tissue Distribution

Substances

  • Adrenal Cortex Hormones
  • Amyloid beta-Protein Precursor
  • Glucocorticoids
  • Aldosterone
  • Dexamethasone