Cell-autonomous regulation of cell and organ growth in Drosophila by Akt/PKB

Nat Cell Biol. 1999 Dec;1(8):500-6. doi: 10.1038/70293.

Abstract

Organismal size is determined by a tightly regulated mechanism that coordinates cell growth, cell proliferation and cell death. The Drosophila insulin receptor/Chico/Dp110 pathway regulates cell and organismal size. Here we show that genetic manipulation of the phosphoinositide-3-OH-kinase-dependent serine/threonine protein kinase Akt (protein kinase B) during development of the Drosophila imaginal disc affects cell and organ size in an autonomous manner. Ectopic expression of Akt does not affect cell-fate determination, apoptosis or proliferation rates in imaginal discs. Thus, Akt appears to stimulate intracellular pathways that specifically regulate cell and compartment size independently of cell proliferation in vivo.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Apoptosis
  • Cell Count
  • Cell Differentiation
  • Cell Division
  • Cell Line
  • Cell Lineage
  • Cell Size
  • Clone Cells / cytology
  • Clone Cells / drug effects
  • Clone Cells / enzymology
  • Clone Cells / metabolism
  • Drosophila Proteins
  • Drosophila melanogaster / cytology*
  • Drosophila melanogaster / embryology*
  • Drosophila melanogaster / enzymology
  • Drosophila melanogaster / genetics
  • Enzyme Activation / drug effects
  • Eye / cytology
  • Eye / embryology
  • Eye / enzymology
  • Eye / metabolism
  • Flow Cytometry
  • Insulin / pharmacology
  • Kinetics
  • Phenotype
  • Phosphatidylinositol 3-Kinases / metabolism
  • Phosphoinositide-3 Kinase Inhibitors
  • Protein-Serine-Threonine Kinases*
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-akt
  • Receptor, Insulin / metabolism
  • Transformation, Genetic
  • Wings, Animal / cytology
  • Wings, Animal / embryology
  • Wings, Animal / enzymology
  • Wings, Animal / metabolism

Substances

  • Drosophila Proteins
  • Insulin
  • Phosphoinositide-3 Kinase Inhibitors
  • Proto-Oncogene Proteins
  • Receptor, Insulin
  • Akt1 protein, Drosophila
  • Protein-Serine-Threonine Kinases
  • Proto-Oncogene Proteins c-akt