RNA editing sites in tobacco chloroplast transcripts: editing as a possible regulator of chloroplast RNA polymerase activity

Mol Gen Genet. 1999 Oct;262(3):462-7. doi: 10.1007/s004380051106.

Abstract

Genetic information in chloroplast DNA is sometimes altered at the transcript level by a process known as RNA editing. Sequence analysis of amplified cDNAs for 69 potential editing sites revealed 13 real editing sites in transcripts of 11 tobacco chloroplast genes. Together with those reported previously, these bring the total of edited sites observed in tobacco chloroplast transcripts to 31 (all involve C to U conversion). Alignment of sequences around the 31 editing sites revealed no obvious consensus, apart from an apparent bias for U or C at position -1 and A at position +2. Editing in tobacco rpoA mRNA restores the conserved leucine residue which is known to be important for transcriptional activation of the alpha subunit of E. coli RNA polymerase. Editing of this site is partial and the extent of editing depends on developmental conditions, suggesting that editing is, at least in part, involved in the regulation of chloroplast-encoded RNA polymerase activity.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chloroplasts / enzymology
  • Chloroplasts / genetics*
  • DNA-Directed RNA Polymerases / metabolism*
  • Gene Expression Regulation, Enzymologic*
  • Molecular Sequence Data
  • Plants, Toxic*
  • RNA Editing*
  • RNA, Plant / metabolism*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Tobacco / enzymology
  • Tobacco / genetics*
  • Zea mays / genetics

Substances

  • RNA, Plant
  • DNA-Directed RNA Polymerases
  • RNA polymerase alpha subunit