In vitro immune function after vaccination with an inactivated, gp120-depleted HIV-1 antigen with immunostimulatory oligodeoxynucleotides

Vaccine. 2000 Jan 6;18(11-12):1081-7. doi: 10.1016/s0264-410x(99)00368-0.


We examined the effect of a synthetic oligodeoxynucleotide containing unmethylated CpG immunostimulatory sequences (ISS) as an adjuvant for an HIV-1 immunogen (inactivated, gp120-depleted HIV-1 virus particles). The addition of the ISS to HIV-1 in incomplete Freund's adjuvant (IFA) was the optimal combination for the production of HIV-1-specific immune responses as measured by IFN-gamma (p=0.002) and IgG antibody production. Furthermore, the group that was immunized with HIV/IFA/ISS, as well as the group that received HIV-1 in complete Freund's adjuvant (CFA), stimulated significantly more RANTES (a beta-chemokine) (p=0.002) production from lymph node cells. These results suggest that the addition of CpG immunostimulatory sequences to HIV antigens in IFA may optimize HIV-1-specific immune responses and provides further rationale for the testing of ISS in combination with gp120-depleted whole-killed HIV-1 in IFA as a potential preventive or therapeutic HIV-1 vaccine.

MeSH terms

  • AIDS Vaccines / immunology*
  • Adjuvants, Immunologic / pharmacology*
  • Animals
  • Chemokine CCL5 / biosynthesis
  • Dinucleoside Phosphates / pharmacology*
  • HIV Antibodies / blood
  • HIV Antigens / immunology*
  • HIV Core Protein p24 / immunology
  • HIV-1 / immunology*
  • Interferon-gamma / biosynthesis
  • Lymph Nodes / metabolism
  • Oligodeoxyribonucleotides / pharmacology*
  • Rats
  • Rats, Inbred Lew
  • Vaccination
  • Vaccines, Inactivated / immunology


  • AIDS Vaccines
  • Adjuvants, Immunologic
  • Chemokine CCL5
  • Dinucleoside Phosphates
  • HIV Antibodies
  • HIV Antigens
  • HIV Core Protein p24
  • Oligodeoxyribonucleotides
  • Vaccines, Inactivated
  • cytidylyl-3'-5'-guanosine
  • Interferon-gamma