Dendritic cells require T cells for functional maturation in vivo

Immunity. 1999 Nov;11(5):625-36. doi: 10.1016/s1074-7613(00)80137-5.

Abstract

We examined dendritic cell (DC) status in SCID and RAG2 -/- mice to assess the influence of T cells on DC development and function in vivo. These mice have reduced numbers of DC in the epidermis and lymph nodes draining hapten-sensitized skin. Epidermal DC in these mice were defective in presenting antigen in vivo to adoptively transferred, hapten-sensitized T cells from normal mice. Likewise, draining lymph node DC were deficient in their capacity to stimulate naive T cells in vitro and in vivo. DC numbers as well as the impaired ability to present antigen in vivo, were corrected by reconstituting these animals with normal T lymphocytes, suggesting that T cells are crucial for normal DC maturation and function in vivo.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adoptive Transfer
  • Animals
  • Antigen Presentation
  • Cell Count
  • Cell Differentiation
  • DNA-Binding Proteins / deficiency
  • DNA-Binding Proteins / genetics
  • Dendritic Cells / cytology*
  • Dermatitis, Allergic Contact / immunology
  • Dermatitis, Allergic Contact / pathology
  • Epidermis / immunology
  • Epidermis / pathology
  • Haptens / immunology
  • Langerhans Cells / pathology
  • Lymph Nodes / immunology
  • Lymph Nodes / pathology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, SCID
  • T-Lymphocyte Subsets / physiology*
  • T-Lymphocyte Subsets / transplantation

Substances

  • DNA-Binding Proteins
  • Haptens
  • Rag2 protein, mouse
  • V(D)J recombination activating protein 2