The temporal requirement for endothelin receptor-B signalling during neural crest development

Nature. 1999 Dec 2;402(6761):496-501. doi: 10.1038/990040.


Endothelin receptor B (EDNRB) is a G-protein-coupled receptor with seven transmembrane domains which is required for the development of melanocytes and enteric neurons. Mice that are homozygous for a null mutation in the Ednrb gene are almost completely white and die as juveniles from megacolon. To determine when EDNRB signalling is required during embryogenesis, we have exploited the tetracycline-inducible system to generate strains of mice in which the endogenous Ednrb locus is under the control of the tetracycline-dependant transactivators tTa or rtTA. By using this system to express Ednrb at different stages of embryogenesis, we have determined that EDNRB is required during a restricted period of neural crest development between embryonic days 10 and 12.5. Moreover, our results imply that EDNRB is required for the migration of both melanoblasts and enteric neuroblasts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cecum / cytology
  • Cecum / embryology
  • Cell Differentiation / genetics
  • Cell Movement / physiology
  • Colon / cytology
  • Colon / embryology
  • Doxycycline / pharmacology
  • Gene Expression Regulation, Developmental / drug effects
  • Melanocytes / cytology
  • Mice
  • Mice, Inbred C57BL
  • Neural Crest / metabolism*
  • Neurons / cytology
  • Receptor, Endothelin B
  • Receptors, Endothelin / genetics
  • Receptors, Endothelin / metabolism
  • Receptors, Endothelin / physiology*
  • Signal Transduction*
  • Tetracycline / pharmacology
  • Trans-Activators / metabolism


  • Receptor, Endothelin B
  • Receptors, Endothelin
  • Trans-Activators
  • Tetracycline
  • Doxycycline