Epstein-Barr Virus Envelope Glycoprotein gp350 Induces NF-kappaB Activation and IL-1beta Synthesis in Human Monocytes-Macrophages Involving PKC and PI3-K

FASEB J. 1999 Dec;13(15):2203-13. doi: 10.1096/fasebj.13.15.2203.


Epstein-Barr virus (EBV) is a highly immunotropic human herpesvirus with oncogenic potential and is involved in numerous pathologies. EBV utilizes its major envelope glycoprotein gp350 to bind to its receptor CR2/CD21 on target cells for initiating the infection. We have previously shown that EBV is able to modulate transcription and translation of a number of cytokine genes via its gp350-mediated binding to this receptor. However, the effects of the binding of purified gp350 to CR2/CD21 on plastic-adherent monocyte-macrophages (AMM) have not been investigated. These cells are a rich source of potent proinflammatory and immune-modulating cytokines, and express low levels of CR2/CD21. We show here for the first time that recombinant gp350 (rgp350) causes production of the potent proinflammatory cytokine IL-1beta in human AMM. Surprisingly, rgp350 is comparable in this capacity to the phorbol ester 12-0-tetradecanoylphorbol 13-acetate. This induction of IL-1beta production was accompanied by increased steady-state levels of its mRNA in gp350-treated AMM, and was dependent on the specific binding of rgp350 to the EBV receptor CR2/CD21. We also show that the signaling pathways resulting in the induction of IL-1beta synthesis by rgp350 required protein kinase C and phosphatidylinositol 3,4,5 triphosphate kinase activities and occurred via activation of the NF-kappaB family of transcription factors.-D'Addario, M., Ahmad, A., Xu, J. W., Menezes, J. Epstein-Barr virus envelope glycoprotein gp350 induces NF-kappaB activation and IL-1beta synthesis in human monocytes-macrophages involving PKC and PI3-K.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Gene Expression Regulation
  • Herpesvirus 4, Human
  • Humans
  • In Vitro Techniques
  • Interleukin-1 / biosynthesis*
  • Interleukin-1 / genetics
  • Macrophages / enzymology
  • Macrophages / metabolism*
  • Macrophages / virology
  • Monocytes / enzymology
  • Monocytes / metabolism*
  • Monocytes / virology
  • NF-kappa B / antagonists & inhibitors
  • NF-kappa B / metabolism*
  • Phosphatidylinositol 3-Kinases / metabolism*
  • Protein Kinase C / metabolism*
  • RNA, Messenger / biosynthesis
  • Receptors, Complement 3d / immunology
  • Signal Transduction
  • Viral Matrix Proteins / pharmacology*


  • Antibodies, Monoclonal
  • EBV-associated membrane antigen, Epstein-Barr virus
  • Interleukin-1
  • NF-kappa B
  • RNA, Messenger
  • Receptors, Complement 3d
  • Viral Matrix Proteins
  • Phosphatidylinositol 3-Kinases
  • Protein Kinase C