Genome-wide analysis of gene expression regulated by the yeast cell wall integrity signalling pathway

Mol Microbiol. 1999 Dec;34(5):1049-57. doi: 10.1046/j.1365-2958.1999.01667.x.


The cell integrity pathway of Saccharomyces cerevisiae monitors cell wall remodelling during growth and differentiation. Additionally, this pathway responds to environmental stresses that challenge the integrity of the cell wall. We conducted a genome-wide survey of genes whose expression was altered in response to activation of Mpk1/Slt2, the MAP kinase, under the control of cell integrity signalling. We identified 25 genes whose regulation was altered by Mpk1 activity. Among these, 20 were positively regulated by Mpk1, and five were negatively regulated. Most of the genes identified encode either known or suspected cell wall proteins or enzymes involved in cell wall biogenesis. These include glycosyl-phosphatidylinositol (GPI) proteins, members of the Pir family of cell wall proteins, Mpk1 itself and others. All of the regulation detected was mediated by the Rlm1 transcription factor, a MADS-box protein that is phosphorylated and activated by Mpk1, but for which no transcriptional targets had been identified. A similar pattern of regulation was observed when cell integrity signalling was induced by environmental stress (i.e. temperature upshift).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Wall / metabolism*
  • Enzyme Activation
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Fungal*
  • Glycosylphosphatidylinositols
  • MADS Domain Proteins
  • Mitogen-Activated Protein Kinases*
  • Nucleic Acid Hybridization / methods
  • RNA, Fungal / genetics
  • RNA, Fungal / isolation & purification
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Signal Transduction*
  • Temperature
  • Transcription Factors / genetics
  • Transcription Factors / metabolism


  • Fungal Proteins
  • Glycosylphosphatidylinositols
  • MADS Domain Proteins
  • RLM1 protein, S cerevisiae
  • RNA, Fungal
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Mitogen-Activated Protein Kinases
  • SLT2 protein, S cerevisiae