Regulation of Gelatin-Binding Protein 28 (GBP28) Gene Expression by C/EBP

Biol Pharm Bull. 1999 Nov;22(11):1158-62. doi: 10.1248/bpb.22.1158.

Abstract

We have previously reported the isolation of human gelatin-binding protein 28 (GBP28) gene which is specifically expressed in adipose tissue. The transcriptional activity of the flanking region of the GBP28 gene was examined by the transient transfection of promoter-luciferase reporter constructs into 3T3 adipocytes and electrophoretic mobility shift assay. This revealed the existence of a protein which binds to the 5'-flanking region of the GBP28 gene in nuclear extracts from human adipose tissue, but not in nuclear extracts from mouse liver. The C/EBP sites contained in this region are thought to take part in the regulation of GBP28 gene expression.

MeSH terms

  • 3T3 Cells
  • Adiponectin
  • Adipose Tissue / metabolism
  • Animals
  • Base Sequence
  • CCAAT-Enhancer-Binding Proteins
  • Carrier Proteins / biosynthesis*
  • Carrier Proteins / genetics*
  • Cell Nucleus / physiology
  • Cells, Cultured
  • DNA-Binding Proteins / genetics*
  • Electrophoresis, Polyacrylamide Gel
  • Gene Expression Regulation / genetics*
  • Humans
  • Indicators and Reagents
  • Intercellular Signaling Peptides and Proteins*
  • Liver / metabolism
  • Luciferases / biosynthesis
  • Luciferases / genetics
  • Mice
  • Molecular Sequence Data
  • Nuclear Proteins / genetics*
  • Plasmids / genetics
  • Promoter Regions, Genetic / genetics
  • Proteins*
  • Terminal Repeat Sequences
  • Transcription Factors / genetics*
  • Transfection / genetics

Substances

  • Adiponectin
  • CCAAT-Enhancer-Binding Proteins
  • Carrier Proteins
  • DNA-Binding Proteins
  • Indicators and Reagents
  • Intercellular Signaling Peptides and Proteins
  • Nuclear Proteins
  • Proteins
  • Transcription Factors
  • Luciferases