Quantitative trait locus mapping of airway responsiveness to chromosomes 6 and 7 in inbred mice

Am J Physiol. 1999 Dec;277(6):L1118-23. doi: 10.1152/ajplung.1999.277.6.L1118.


Quantitative trait locus (QTL) mapping was used to identify chromosomal regions contributing to airway hyperresponsiveness in mice. Airway responsiveness to methacholine was measured in A/J and C3H/HeJ parental strains as well as in progeny derived from crosses between these strains. QTL mapping of backcross [(A/J x C3H/HeJ) x C3H/HeJ] progeny (n = 137-227 informative mice for markers tested) revealed two significant linkages to loci on chromosomes 6 and 7. The QTL on chromosome 6 confirms the previous report by others of a linkage in this region in the same genetic backgrounds; the second QTL, on chromosome 7, represents a novel locus. In addition, we obtained suggestive evidence for linkage (logarithm of odds ratio = 1.7) on chromosome 17, which lies in the same region previously identified in a cross between A/J and C57BL/6J mice. Airway responsiveness in a cross between A/J and C3H/HeJ mice is under the control of at least two major genetic loci, with evidence for a third locus that has been previously implicated in an A/J and C57BL/6J cross; this indicates that multiple genetic factors control the expression of this phenotype.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Asthma / genetics*
  • Bronchial Hyperreactivity / chemically induced
  • Bronchial Hyperreactivity / genetics*
  • Bronchial Provocation Tests
  • Bronchoconstrictor Agents
  • Chromosome Mapping*
  • Chromosomes
  • Genetic Linkage
  • Genotype
  • Male
  • Methacholine Chloride
  • Mice
  • Mice, Inbred A
  • Mice, Inbred C3H
  • Quantitative Trait, Heritable


  • Bronchoconstrictor Agents
  • Methacholine Chloride