Conditional sigma factor expression, using the inducible acetamidase promoter, reveals that the Mycobacterium tuberculosis sigF gene modulates expression of the 16-kilodalton alpha-crystallin homologue

J Bacteriol. 1999 Dec;181(24):7629-33. doi: 10.1128/JB.181.24.7629-7633.1999.

Abstract

A chemically inducible acetamidase promoter-sigF fusion gene was integrated into the chromosome of Mycobacterium bovis BCG. Two-dimensional protein gel analysis permitted the identification of a number of protein spots whose expression was SigF related. One spot upregulated by inappropriate induction of sigF expression corresponded to the 16-kDa antigen alpha-crystallin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amidohydrolases / genetics*
  • Antigens, Bacterial / biosynthesis*
  • Antigens, Bacterial / genetics
  • Bacterial Proteins* / genetics*
  • Chromosomes, Bacterial
  • Electrophoresis, Gel, Two-Dimensional
  • Enzyme Induction / genetics
  • Gene Expression Regulation, Bacterial*
  • Mycobacterium tuberculosis / genetics*
  • Sigma Factor* / biosynthesis*
  • Sigma Factor* / genetics*
  • Up-Regulation

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • FliA protein, Bacteria
  • Sigma Factor
  • Mycobacterium tuberculosis antigens
  • Amidohydrolases
  • acetamidase