Inhibitory effect of pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters

FEBS Lett. 1999 Dec 10;463(1-2):53-7. doi: 10.1016/s0014-5793(99)01584-7.

Abstract

Pax4 is a paired-box transcription factor that plays an important role in the development of pancreatic beta-cells. Two Pax4 cDNAs were isolated from a rat insulinoma library. One contained the full-length sequence of Pax4. The other, termed Pax4c, was identical to Pax4 but lacked the sequences encoding 117 amino acids at the COOH-terminus. Pax4 was found to inhibit the human insulin promoter through a sequence element, the C2 box, located at -253 to -244, and the islet amyloid polypeptide promoter through a sequence element located downstream of -138. The inhibitory activity of Pax4 was mapped to separate regions of the protein between amino acids 2-230 and 231-349.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid / antagonists & inhibitors*
  • Amyloid / genetics
  • Animals
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Gene Library
  • Homeodomain Proteins / genetics*
  • Homeodomain Proteins / physiology*
  • Humans
  • Insulin Antagonists*
  • Islet Amyloid Polypeptide
  • Islets of Langerhans / metabolism
  • Mice
  • Paired Box Transcription Factors
  • Plasmids
  • Promoter Regions, Genetic*
  • Protein Biosynthesis
  • Protein Isoforms
  • Rats
  • Transcription Factors / genetics*
  • Transcription Factors / physiology*
  • Transfection

Substances

  • Amyloid
  • DNA, Complementary
  • Homeodomain Proteins
  • Insulin Antagonists
  • Islet Amyloid Polypeptide
  • PAX4 protein, human
  • Paired Box Transcription Factors
  • Pax4 protein, mouse
  • Pax4 protein, rat
  • Protein Isoforms
  • Transcription Factors

Associated data

  • GENBANK/AF198155
  • GENBANK/AF198156