Systemic anti-IFN-gamma treatment and role of macrophage subsets in the foreign body reaction to dermal sheep collagen in rats

J Biomed Mater Res. 2000 Mar 5;49(3):297-304. doi: 10.1002/(sici)1097-4636(20000305)49:3<297::aid-jbm1>;2-0.


The application of a biomaterial induces a foreign body reaction. By controlling this reaction, biocompatibility could be improved. We previously demonstrated that impregnation of a biodegradable biomaterial with antibodies against interferon-gamma (IFN-gamma) inhibits the foreign body reaction. In this study we investigate whether systemic administration of the antibody can induce similar reactions. Several parameters are compared between control and anti-IFN-gamma-treated rats: cellular ingrowth; degradation of the biomaterial; ingrowth of macrophage (MO) subsets, T cells, B cells, NK cells, and granulocytes; and expression of the major histocompatibility complex class II (MHC class II) molecule on antigen presenting cells. Treatment with anti-IFN-gamma results in increased cellular ingrowth and biomaterial degradation and a decreased expression of MHC class II. Overall, systemic treatment with anti-IFN-gamma is insufficient to modulate the foreign body reaction. This suggests an alternative mechanism for MO activation besides IFN-gamma. The role of T cells and MO subsets in the foreign body reaction is discussed.

MeSH terms

  • Animals
  • Antibodies / administration & dosage
  • Biocompatible Materials / toxicity*
  • Collagen / immunology
  • Collagen / toxicity*
  • Foreign-Body Reaction / immunology
  • Foreign-Body Reaction / pathology
  • Foreign-Body Reaction / prevention & control*
  • Interferon-gamma / antagonists & inhibitors*
  • Macrophage Activation
  • Macrophages / classification
  • Macrophages / immunology*
  • Macrophages / pathology
  • Male
  • Rats
  • Sheep
  • Skin / immunology


  • Antibodies
  • Biocompatible Materials
  • Interferon-gamma
  • Collagen