The mouse adducin gene family: alternative splicing and chromosomal localization

Mamm Genome. 2000 Jan;11(1):16-23. doi: 10.1007/s003350010004.


Mouse cDNA sequences encoding alpha, beta, and gamma adducins were cloned from a mouse reticulocyte cDNA library. The purified clones contain alternatively spliced exons from all three adducin genes. In the case of alpha and beta, the inclusion of the alternatively spliced exons results in truncated polypeptide isoforms (called alpha-2 and beta-2). The mouse predicted amino acid sequences are compared with published rat and human sequences. For completion of this comparison, cDNA encoding the rat beta-1 carboxy terminus was cloned by PCR. The carboxy terminal region containing MARCKS homology, calmodulin-binding region-2, and spectrin-actin-binding site, is conserved among alpha-1, beta-1, and gamma-1 isoforms in mouse, rat, and humans. We also report here the localization of the gene encoding gamma adducin (Add3) to murine Chr 19, in a region that shows conserved synteny with human Chr 10.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing / genetics*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Calmodulin-Binding Proteins / genetics*
  • Chromosome Mapping*
  • Cloning, Molecular
  • Cytoskeletal Proteins / genetics
  • DNA Primers / chemistry
  • DNA, Complementary / chemistry
  • Exons / genetics
  • Gene Library
  • Humans
  • Mice
  • Molecular Sequence Data
  • Rats
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Tumor Cells, Cultured


  • Calmodulin-Binding Proteins
  • Cytoskeletal Proteins
  • DNA Primers
  • DNA, Complementary
  • adducin

Associated data

  • GENBANK/AF096839
  • GENBANK/AF100422
  • GENBANK/AF100423
  • GENBANK/AF100424
  • GENBANK/AF100425
  • GENBANK/AF130338