Genomic cloning, chromosomal mapping, and expression analysis of msal-2

Mamm Genome. 2000 Jan;11(1):64-8. doi: 10.1007/s003350010012.


Mutations of SALL1 related to spalt of Drosophila have been found to cause Townes-Brocks syndrome, suggesting a function of SALL1 for the development of anus, limbs, ears, and kidneys. No function is yet known for SALL2, another human spalt-like gene. The structure of SALL2 is different from SALL1 and all other vertebrate spalt-like genes described in mouse, Xenopus, and Medaka, suggesting that SALL2-like genes might also exist in other vertebrates. Consistent with this hypothesis, we isolated and characterized a SALL2 homologous mouse gene, Msal-2. In contrast to other vertebrate spalt-like genes both SALL2 and Msal-2 encode only three double zinc finger domains, the most carboxyterminal of which only distantly resembles spalt-like zinc fingers. The evolutionary conservation of SALL2/Msal-2 suggests that two lines of sal-like genes with presumably different functions arose from an early evolutionary duplication of a common ancestor gene. Msal-2 is expressed throughout embryonic development but also in adult tissues, predominantly in brain. However, the function of SALL2/Msal-2 still needs to be determined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Chromosome Mapping*
  • Cloning, Molecular
  • DNA / chemistry
  • DNA Primers / chemistry
  • DNA-Binding Proteins
  • Gene Expression Regulation, Developmental*
  • Gene Library
  • In Situ Hybridization, Fluorescence
  • Intracellular Signaling Peptides and Proteins*
  • Mice
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • RNA / chemistry
  • RNA / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcription Factors
  • Zinc Fingers / genetics*


  • Carrier Proteins
  • DNA Primers
  • DNA-Binding Proteins
  • Intracellular Signaling Peptides and Proteins
  • Sall2 protein, mouse
  • Transcription Factors
  • RNA
  • DNA

Associated data

  • GENBANK/AJ007396