A splice variant of human ephrin-A4 encodes a soluble molecule that is secreted by activated human B lymphocytes

Blood. 2000 Jan 1;95(1):221-30.


Ephrin-A4 is a ligand for the erythropoietin-producing hepatocellular (Eph) receptor family of tyrosine kinases. We have identified a secreted form of ephrin-A4, denoted ephrin-A4 (s), which is encoded by an alternatively spliced mRNA and is produced by in vivo activated B cells in tonsils. Blood B cells secrete ephrin-A4 (s) upon stimulation via the B-cell antigen receptor. A subpopulation of tonsil cells in the crypts with a dendritic cell phenotype was shown to express EphA2, an Eph receptor tyrosine kinase that was found to be capable of binding an ephrin-A4 immunoglobulin chimeric protein. We conclude that ephrin-A4 (s) may play a role in the interaction between activated B lymphocytes and dendritic cells in human tonsils. (Blood. 2000;95:221-230)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing*
  • Amino Acid Sequence
  • B-Lymphocytes / immunology
  • B-Lymphocytes / physiology*
  • Base Sequence
  • Cell Line
  • Cells, Cultured
  • Cloning, Molecular
  • Ephrin-A4
  • Exons
  • Genetic Variation*
  • Glycosylphosphatidylinositols / metabolism
  • Humans
  • In Situ Hybridization
  • Introns
  • Jurkat Cells
  • Lymphocyte Activation*
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics*
  • Molecular Sequence Data
  • Palatine Tonsil / immunology
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcription, Genetic*


  • Ephrin-A4
  • Glycosylphosphatidylinositols
  • Membrane Proteins
  • Recombinant Fusion Proteins

Associated data

  • GENBANK/AJ006352
  • GENBANK/AJ006353