Type 2 cytokine gene activation and its relationship to extent of disease in patients with tuberculosis

J Infect Dis. 2000 Jan;181(1):385-9. doi: 10.1086/315200.


The extent of type 2 cytokine gene expression in patients with pulmonary tuberculosis (TB) was studied by use of quantitative nested reverse-transcription polymerase chain reaction on freshly isolated peripheral blood mononuclear cells. Interleukin (IL)-4 and IL-13 mRNA expression was significantly greater in patients-median mRNA copy numbers were 1.7 and 1.1 log10 higher, respectively-than in matched tuberculin-positive control subjects. Significant correlations with radiologic extent of disease and serum IgE levels supported the biologic significance of these results. Interferon-gamma mRNA copy numbers exceeded those of type 2 cytokines but were only marginally lower in patients than in control subjects. Gene expression of an IL-4 splice variant (IL-4delta2) was bimodally distributed in both patient and control groups. Patients with greater IL-4delta2 expression also expressed more IL-4 mRNA and had more extensive disease. Type 2 cytokines are associated with immunopathologic changes in TB patients but could be a cause or consequence of disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Alternative Splicing
  • Gene Expression Regulation
  • Humans
  • Interferon-gamma / genetics
  • Interleukin-13 / genetics*
  • Interleukin-4 / genetics*
  • Middle Aged
  • RNA, Messenger / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcriptional Activation
  • Tuberculosis, Pulmonary / immunology*
  • Tuberculosis, Pulmonary / pathology


  • Interleukin-13
  • RNA, Messenger
  • Interleukin-4
  • Interferon-gamma