Large-scale monitoring of host cell gene expression during HIV-1 infection using cDNA microarrays

Virology. 2000 Jan 5;266(1):8-16. doi: 10.1006/viro.1999.0044.

Abstract

Human immunodeficiency virus type 1 (HIV-1) infection alters the expression of host cell genes at both the mRNA and protein levels. To obtain a more comprehensive view of the global effects of HIV infection of CD4-positive T-cells at the mRNA level, we performed cDNA microarray analysis on approximately 1500 cellular cDNAs at 2 and 3 days postinfection (p.i.) with HIV-1. Host cell gene expression changed little at 2 days p.i., but at 3 days p.i. 20 cellular genes were identified as differentially expressed. Genes involved in T-cell signaling, subcellular trafficking, and transcriptional regulation, as well as several uncharacterized genes, were among those whose mRNAs were differentially regulated. These results support the hypothesis that HIV-1 infection alters expression of a broad array of cellular genes and provides a framework for future functional studies on the differentially expressed mRNA products.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • CD4-Positive T-Lymphocytes / metabolism*
  • CD4-Positive T-Lymphocytes / pathology
  • CD4-Positive T-Lymphocytes / virology*
  • Cell Line
  • DNA, Complementary
  • Gene Expression Profiling
  • Gene Expression Regulation*
  • HIV-1 / physiology*
  • Humans
  • Image Processing, Computer-Assisted
  • Oligonucleotide Array Sequence Analysis / methods*
  • RNA, Messenger / metabolism
  • Transcription, Genetic

Substances

  • DNA, Complementary
  • RNA, Messenger